Reporter

Part:BBa_K660000:Design

Designed by: Glasgow Uni 2011   Group: iGEM11_Glasgow   (2011-09-16)

LOV2 Domain


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 312
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 312
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 9
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 312
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 312
    Illegal AgeI site found at 287
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 32



Source

The LOV domain was originally isolated from Arabidopsis and has been codon optimised for expression in E.coli.

References